Comparison of localization of gene expression to joint using different serotypes of AAV: in vivo bioluminescence imaging. Wild-type mice received AAV. Luciferase vector packaged in capsid from serotypes AAV2, AAV5, and AAV8 at the dose of 8 × 108 vg/animal by IA injection to the left hind limb knee joint. Bioluminescence imaging with a CCD camera (IVIS, Xenogen) was initiated and recorded exactly 15 minutes after injection of D-luciferin, the substrate of luciferase. After acquiring a grayscale photograph, a bioluminescent image was captured with adjusted exposure time, binning (resolution) factor, 1/f stop and open filter to acquire maximum signal while avoiding a saturating image. (Top panel) One week after vector delivery. (Bottom panel) Four weeks after vector delivery.
