In vivo administration of the CXCR4 antagonist AMD-3100 promotes mobilization of iNK and mNK cells from the BM. Six- to 10-week-old C57BL/6 mice were subcutaneously injected with 50 μL PBS alone (−) or containing 2 mg/mL AMD-3100 and then killed at 60 minutes and at 60 or 180 minutes, respectively, after treatment. Cells from BM, spleen (Sp), and blood (Bl) cells were counted and stained either with anti-CD122, anti-CD19, anti-CD3ϵ, anti-NK1.1, and DX5 or with anti-CD3ϵ, anti-NK1.1, anti-CD11b, and DX5 mAbs to determine the percentage of pNK (C) and iNK/mNK (D-F) cells, respectively. Each dot represents the total cell number in the indicated organ of individual mice from at least 4 independent experiments performed and was calculated as described in “Methods.” Student t test was performed to compare the tissue distribution of NK cell subsets in mice treated with vehicle control with that of mice treated with AMD-3100. *P < .05. Differences that are not statistically significant are omitted for sake of simplicity. §Cell number × 105.
