In vivo administration of CCL3 selectively promotes mobilization of mNK cells from the BM. Six- to 10-week-old C57BL/6 mice were subcutaneously injected with 50 μL PBS alone (−) or containing 20 μg/mL CCL3. Mice treated with PBS or with CCL3 were killed at 30 minutes and at 30 or 180 minutes, respectively, after treatment. Cells from BM, spleen (Sp), and blood (Bl) were counted and stained either with anti-CD122, anti-CD19, anti-CD3ϵ, anti-NK1.1, and DX5 antibodies or with anti-CD3ϵ, anti-NK1.1, anti-CD11b, and DX5 mAbs. Each dot represents the total cell number in the indicated organ of individual mice from at least 4 independent experiments performed and was calculated as described in “Methods.” Student t test was performed to compare the tissue distribution of NK cell subsets in mice treated with vehicle control with that of mice treated with CCL3. *P < .05. Differences that are not statistically significant are omitted for sake of simplicity. §Cell number × 105.
