American Society of Hematology

Figure 1

$Figure 1. The B-cell phenotype in WASp-deficient mice. (A) BM was stained for expression of B220, CD43, IgM, BP1, CD24, CD19, and IgD and developmental stages determined according to the Hardy classification. Absolute numbers for each fraction (Fr) A through F are shown in WT versus WASp−/− mice (ko) (n = 12). (B,C) Splenocytes were stained for expression of B220, CD19, CD24, CD21, CD23, and CD1d and B-cell subsets analyzed. (B) Representative FACS blot gated on B220+CD19+ cells in a WT versus WASp−/− mouse. (C) Absolute numbers of splenic B-cell subsets from WT versus WASp−/− mice (n = 12). (D,E) Peritoneal B cells were stained for IgM, CD5, and Mac1. (D) Representative FACS blot of gating B1 cells in a WT and a WASp−/− mouse. (E) Absolute numbers of B1, B1a, and B1b cells in WT (n = 8) versus WASp−/− mice (n = 7).$

The B-cell phenotype in WASp-deficient mice. (A) BM was stained for expression of B220, CD43, IgM, BP1, CD24, CD19, and IgD and developmental stages determined according to the Hardy classification. Absolute numbers for each fraction (Fr) A through F are shown in WT versus WASp^−/− mice (ko) (n = 12). (B,C) Splenocytes were stained for expression of B220, CD19, CD24, CD21, CD23, and CD1d and B-cell subsets analyzed. (B) Representative FACS blot gated on B220⁺CD19⁺ cells in a WT versus WASp^−/− mouse. (C) Absolute numbers of splenic B-cell subsets from WT versus WASp^−/− mice (n = 12). (D,E) Peritoneal B cells were stained for IgM, CD5, and Mac1. (D) Representative FACS blot of gating B1 cells in a WT and a WASp^−/− mouse. (E) Absolute numbers of B1, B1a, and B1b cells in WT (n = 8) versus WASp^−/− mice (n = 7).

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