Real-time RT-PCR analysis of miRNA expression in splenic lymphocytes. The expression level of selected estrogen-regulated miRNA in freshly isolated splenic lymphocytes between placebo- and estrogen-treated mice were further quantified using Taqman miRNA assays. (A,B) The graphs show miRNA that were confirmed to be significantly up-regulated or down-regulated by estrogen treatment, respectively. Means plus or minus SEM (n ≥ 4 each) are shown in the graphs. *P < .05; **P < .01; and ***P < .001.