DLI from GKO allogeneic donors induces more severe GVHD than WT DLI in preestablished mixed allogeneic chimeras. Lethally (8 Gy) irradiated BALB/c mice were reconstituted with a mixture of TCD BALB/c plus WT (■, n = 8; and ▲, n = 11) or GKO (△, n = 11; and □, n = 10) B6 BMCs 8 weeks before DLI (ie, injection of 1.5 × 10⁷ splenocytes) from WT (■ and △) or GKO (▲ and □) B6 mice. Recipient mice were followed for body weight changes (left) and survival (right). Combined data from 2 similar experiments are shown.