Figure 1
Identification of annexin 1 in human PMN–derived microparticles. Use of multiple detection approaches revealed annexin 1 (AnxA1; 37 kDa) presence in PMN-derived microparticles. (A) Western blot analysis comparing human recombinant AnxA1 (0.1 ng) and extracts from approximately 60 × 103 microparticles; arrow indicates AnxA1 band. (B) Protein gel electrophoresis revealing multiple bands in PMN-derived microparticles. Bands in the approximately 37-kDa region were excised, digested, and subjected to LC/MS/MS where both the MASCOT scores and the RANDOM database scores confirm the presence of this protein; boxed amino acid sequences indicate the fragments identified from this analysis. Data are representative of 4 distinct analyses.
