Figure 5
Role for annexin 1 in the inhibitory property of PMN-derived microparticles. PMNs (5 × 106) were incubated with microparticles (between 30 and 50 × 103) for 8 minutes at 37°C. In some tubes, isotype control Ab, or neutralizing anti-AnxA1 or anti-ALX mAb was also added. PMNs (5 × 106) were then flown for 15 minutes at 1 dyne/cm2, prior to quantifying the degree of PMN interaction with the HUVECs, as (A) PMN capture, (B) PMN rolling, or (C) PMN adhesion. Data are mean plus or minus SEM of from 4 or more independent experiments (with distinct microparticles, PMN, and HUVEC preparations). *P < .05 versus microparticle vehicle group.
