Figure 4
Figure 4. miR-21 or miR-196b is a partial phenocopy of Gfi1−/−. (A) Methylcellulose colony assay from Lin− bone marrow cells transduced with MSCV encoding miR-21 or a nontargeting shRNA control (NT) reveals significantly increased CFU-Ms. (B) Cytospins from the colony assay in panel A. (C) Colony assay from Lin− bone marrow cells treated with control antagomiR or antagomiR-21 reveals significantly decreased CFU-Ms. (D) Methylcellulose colony assay from Lin− bone marrow cells transduced with MSCV encoding miR-196b or a nontargeting control (NT) reveals significantly decreased CFU-Gs. (E) Cytospins from the colony assay in panel D. (F) Colony assay from Lin− bone marrow cells treated with control antagomiR or antagomiR-196b reveals significantly increased CFU-Gs. Results are displayed as percentage of CFU-Gs, CFU-Ms, and CFU-GMs (n = 3, mean ± SD). TaqMan results are represented as means ± SD from at least 3 independent experiments. *P < .05; **P < .01.

miR-21 or miR-196b is a partial phenocopy of Gfi1−/−. (A) Methylcellulose colony assay from Lin− bone marrow cells transduced with MSCV encoding miR-21 or a nontargeting shRNA control (NT) reveals significantly increased CFU-Ms. (B) Cytospins from the colony assay in panel A. (C) Colony assay from Lin− bone marrow cells treated with control antagomiR or antagomiR-21 reveals significantly decreased CFU-Ms. (D) Methylcellulose colony assay from Lin− bone marrow cells transduced with MSCV encoding miR-196b or a nontargeting control (NT) reveals significantly decreased CFU-Gs. (E) Cytospins from the colony assay in panel D. (F) Colony assay from Lin− bone marrow cells treated with control antagomiR or antagomiR-196b reveals significantly increased CFU-Gs. Results are displayed as percentage of CFU-Gs, CFU-Ms, and CFU-GMs (n = 3, mean ± SD). TaqMan results are represented as means ± SD from at least 3 independent experiments. *P < .05; **P < .01.

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