OT-I cells undergoing deletion fail to up-regulate GzmB and Ly6C. A total of 2 × 10⁶ Ly5.1⁺ CFSE labeled OT-I cells were injected intravenously into either RIP-OVA^hi mice or OCS/LPS primed C57BL/6 mice (OCS/LPS). Sixty hours after transfer, the proliferating cells within the sacral and pancreatic lymph nodes (RIP-OVA^hi) or spleen (OCS/LPS) were stained and analyzed by flow cytometry. (A) Dot plots of intracellular GzmB staining versus CFSE staining showing unstained (background; left panels) and GzmB-stained (right panels) cells. The plots are gated on CD8⁺Ly5.1⁺ T cells. Representative plots from 5 independent experiments (each performed with 3 mice per group) are shown. (B) Data for Ly6C staining plotted similarly to panel A. Representative plots from 3 independent experiments (each performed with 3 mice per group) are shown.