Antigen-specific degranulation in CD8⁺ T cells from a HAM/TSP patient. PBMCs of a HAM/TSP patient (HAM#5) were cultured without any stimulation (A,E) or with Tax11-19 peptides (B,F) or CMVpp65 peptides (C,G) for 5 hours. Top or bottom panels show the detection of CD107a with Tax11-19 tetramer and CMV pp65 tetramer staining, respectively. Panels D and H show the results of spontaneous degranulation in CD8⁺ T cells of unstimulated culture for 24 hours.