Characterization of mC7. (A) Western blot analysis of affinity-purified C7 from human normal serum (track 1), from cell supernatant (track 2), or from cell lysate (track 3). (B) Northern blot analysis of mRNA purified from 2 preparations of HUVECs (track 1 and 2), HepG2 (positive control; track 3), and Raji cells (negative control; track 4). (C) Ethidium bromide image of 2 preparations of HUVECs (track 1 and 2), HepG2 (track 3), and Raji cells (track 4). One of 3 experiments with similar results is shown.