TCC assembled on mC7 down-regulates the proinflammatory effects of SC5b-9. (A) Expression of adhesion molecules. HUVECs were stimulated with SC5b-9 either alone or in the presence of the mTCC. The expression of the adhesion molecules was evaluated by ELISA on whole cells using mAbs to vascular adhesion molecule-1 (VCAM-1; 12 hours after stimulation), intercellular adhesion molecule-1 (ICAM-1), or E-selectin (both 4 hours after stimulation). Values are mean (± SD) of triplicate determinations of 3 separate experiments (*P < .05 vs complete cell- culture medium). (B) Synthesis of IL-8. The amount of IL-8 in supernatant of HUVECs stimulated for 12 hours with SC5b-9, in the presence or in the absence of mTCC, or TNF-α, as a positive control, or complete cell-culture medium alone, was evaluated by ELISA. One of 3 experiments with similar results is shown. (C) Endothelial leakage assay. HUVECs grown to confluence on the insert of the Transwell were treated with SC5b-9 in the presence or in the absence of mTCC. The FITC-BSA amount collected in the lower chamber after 30 minutes was measured by the Infinte200 (Tecan, Männedorf, Switzerland). Values are mean (± SD) of triplicate determinations of 4 separate experiments (**P < .01 vs complete cell-culture medium).