SDS-PAGE analysis of αCD30 antibody and huTR variants after purification. SDS-PAGE analysis of αCD30 antibody variants and huTR after protein L (scFv, scFv-RNase) or protein A purification. SDS-PAGE was performed under reducing conditions followed by silver staining (A) or Western blot analysis using RNase specific polyclonal rabbit serum and anti-rabbit IgG AP conjugate (B) or using human Fc-specific IgG AP conjugate (C) for detection.