Flow cytometric analysis of binding to CD30 on lymphoma cells. Top histograms show αCD30scFv-Fc () staining of CD30⁺ Karpas cells (left) and of CD30⁻ HD-MY-Z cells (right). Bottom histograms show TR () staining of CD30⁺ Karpas cells (left) and of CD30⁻ HD-MY-Z cells (right). Bound αCD30scFv-Fc and huTR were detected with anti-human IgG (Fc-specific) antibody FITC conjugate. The chimeric 215-IgG served as negative control (black solid line).