Limited role of TSLP in IL-7–independent BM B lymphopoiesis. (A) FACS profiles (top) and mean (SD) numbers (bottom) of pro-B, pre-B, and imm-B cells in BM of 9- to 11-week-old WT littermate (n = 5) and Tpte2^−/− (n = 8) mice (each from 3 litters). (B) FACS profiles and mean (SD) numbers of B220IgM⁺ cells in spleen of 9- to 11-week-old WT littermate (n = 3) and Tpte2^−/− (n = 13) littermate controls. (C) Pro-B, pre-B, and imm-B cells in BM and (D) B220IgM⁺ cells in spleen of 3-week-old littermate Il7^−/− and Il7^−/−Tpte2^−/− mice (n = 7 and n = 12, 4 litters). Numbers in FACS profiles show the mean percentage of cells within the indicated gates or quadrants of total BM or spleen cells (*P < .05).