Mature B-cell subsets demonstrate distinct miRNA profiles. (A) Overall schema of mature B-cell differentiation. (B) Selection of the B-cell subsets with the use of flow cytometry. Cells were previously gated on CD19⁺ cells. Naive and memory B cells were distinguished from GC and plasma cells based on surface CD38 and IgD expression. (C) Distinction of naive and memory B cells based on IgD and CD27 expression with the use of flow cytometry. (D) Relative expression of miRNA in the naive to GC B-cell transition. miRNAs that were, on average, at least 2-fold differentially expressed at a false discovery rate of less than 5% are shown according to the color scale. (E) Relative expression of mRNA in the naive to GC B-cell transition. mRNAs that were, on average, at least 2-fold differentially expressed at a false discovery rate of less than 1% are shown according to the color scale. (F) Relative expression of miRNA in the GC B-cell to plasma-cell transition. miRNAs that were, on average, at least 2-fold differentially expressed at a false discovery rate of less than 5% are shown according to the color scale. (G) Relative expression of mRNA in the GC B-cell to plasma-cell transition. mRNAs that were, on average, at least 2-fold differentially expressed at a false discovery rate of less than 1% are shown according to the color scale. (H) Relative expression of miRNA in the GC B cell to memory B–cell transition. miRNAs that were, on average, at least 2-fold differentially expressed at a false discovery rate of less than 5% are shown according to the color scale. (I) Relative expression of mRNA in the GC B-cell to memory B–cell transition. mRNAs that were, on average, at least 2-fold differentially expressed at a false discovery rate of less than 1% are shown according to the color scale. (J) Expression of key miRNA processing genes DGCR8, DICER1, EIF2C2, DROSHA, and XPO5 is unchanged among the B-cell subsets (P > .1 in all cases).