Increased infiltration of Th1 cells in liver of recipients given IL-17^−/− donor cells. (A) Liver mononuclear cells from recipients given WT or IL-17^−/− donor cells were stained for H-2^b, CD4, CD8, and intracellular IFN-γ, 13 days after HCT. Gated H-2^b+ cells are shown in IFN-γ versus CD4 or CD8. The percentage of IFN-γ⁺CD4⁺ or CD8⁺ T cells is shown beside the gating boxes. One representative of 4 recipients in each group is shown. (B) Means (± SE) of the percentages of donor IFN-γ⁺ CD4⁺ or CD8⁺ T cells of the 4 recipients and of the yield of IFN-γ⁺ CD4⁺ or CD8⁺ T cells of the 4 recipients. (C) MLN cells of the recipients were stained for H-2^b, CD4, CD8, and CCR5. Histogram of CCR5 (solid line) versus isotype control (shaded area) of H-2^b+ CD4⁺ or CD8⁺ cells are shown. One representative of 4 recipients is shown. Means (± SE) of the percentage of CCR5⁺ cells among CD4⁺ or CD8⁺ donor T cells are 43.1% (± 3.9%) or 10.8% (± 2.0%) in recipients given WT donor cells and 67.6% (± 2.8%) or 23.3% (± 1.6%) in recipients given IL-17^−/− donor cells. (D) Means (± SE) of chemokine expression levels by liver of the recipients given WT or IL-17^−/− donor cells 5 days after HCT. Relative gene expression levels were normalized within each sample to the house keeping gene GAPDH and were presented relative to the expression in liver tissue of syngeneic HCT recipients. There were 4 mice in each group.