Nucleofection of AONs induces specific exon skipping. RT-PCR analysis of RNA isolated from T cells from an IL-12Rβ1^−/− patient and a control 24 hours after nucleofection with various AONs. The primers 1F and 4R are indicated on the right: without exon skipping, a 443-bp product is expected; with exon skipping, a 383-bp product is expected. First lane represents no AON added, with nucleofection; and last lane, water control of the PCR. The size marker is a 100-bp ladder.