Validation of kinome profile results for anti-CD45RB treatment on resting cells. (A) Cells were incubated for various time points with or without anti-CD45RB. The phosphorylation status of Lck, Src, ZAP70, PKB, mTOR, p70S6 kinase, p38, and JNK in whole-cell lysates was assayed using phosphospecific mAbs on Western blot. (B) Cells were incubated for various time points with or without anti-CD3. The phosphorylation status of Lck, Src, ZAP70, PKB, p70S6 kinase, p38, and JNK in whole-cell lysates was assayed using phospho-specific mAbs on Western blot.