Validation of kinome profile results for anti-CD45RB treatments on anti-CD3 activated cells and IL-2 measurements in the presence of various inhibitors. (A) Cells were incubated for various time points with or without anti-CD45RB and simultaneously stimulated with anti-CD3 Ab, and phosphorylation states of the same proteins as in Figure 3 were tested. Actin was used as the loading control. (B) IL-2 was measured 24 hours after CD3 activation and anti-CD45RB mAb treatments in the presence of Src (pp1), p38 (SB203580), and mTOR inhibitors (n = 5). *P < .05.