Suppressive function of CD200R3 expressed on DCs in the Ag-specific CD4⁺ T-cell response. (A) The expression of cell surface molecules on retroviral transfected BM-iDCs was analyzed by flow cytometry, and data are represented by a dot plot. Data are representative of 4 replicate experiments. (B) Rag2^−/−KJ1-26⁺ T cells (5 × 10⁴) were cultured with OVAp/CD200R3⁻GFP⁻BM-iDCs, OVAp/CD200R3⁻GFP⁺BM-iDCs, or OVAp/CD200R3⁺GFP⁺BM-iDCs (6.25 × 10²-5 × 10³) for 3 days, and the proliferative response was measured. * P < .01 compared with OVAp/CD200R3⁻GFP⁻BM-iDCs. Data are mean ± SEM, and the results are combined from 4 replicate experiments.