Phenotypic identification of naturally occurring CD49b⁺ CD200R3⁺ DC_regs. (A) The expression of CD49b and CD200R3 on splenocyes (left panel) and among gated CD49b⁺ cells (right panel) was analyzed by flow cytometry, and data are represented by a dot plot. (B) The expression of FcϵRIα on CD49b⁺CD200R3⁺ cells purified from splenocyes was analyzed by flow cytometry, and data are represented by a dot plot. (C) The expression of CD11c, I-A/I-E, and FcϵRIα among gated FcϵRIα⁻ cells and FcϵRIα⁺ cells in CD49b⁺CD200R3⁺ cells purified from splenocyes was analyzed by flow cytometry, and data are represented by a histogram. Data are representative of 4 replicate experiments (A-C). (D) CD49b and CD200R3 on leukocytes (left panel) and CD49b⁺ cells (right panel) from spleen, peripheral blood, and MLNs depleted of FcϵRIα⁺ cells were analyzed by flow cytometry, and data are percentage positive cells. *P < .01 compared with splenocytes. Data are mean ± SEM, and the results are combined from 4 replicate experiments. (E) The expression of cell surface molecules on CD11c⁺ DCs, CD49b⁺CD200R3⁻ cells, CD49b⁺CD200R3⁺ cells, and BM-DC_regs was analyzed by flow cytometry, and data are represented by a dot plot and a histogram. Data are representative of 4 replicate experiments.