Cancer development in TRAF3 transgenic mice. (A) Percentage of tumors found in wild-type mice (n = 24; 17 ± 1.5 months) and in TRAF3 mice (n = 70; 18 ± 0.8 months). (B) Graphic representation of percentage of mice bearing tumors in the tongue (squamous cell carcinoma, n = 19; papilloma, n = 2), other oral tumors (pleomorphic adenomas, cystadenomas, and adenocarcinomas), lung carcinomas (papillary bronchioloalveolar carcinoma, n = 15; adenoma, n = 2), and hepatocarcinoma (n = 7). (C) Comparative analysis of tongues from 2-month-old (bottom) and 14-month-old (top) TRAF3 mice and wild-type littermates. The arrow indicates the squamous cell carcinoma in the tongue. (D,E) Squamous cell carcinoma of the tongue (H&E). (F,G) Example of lymphoid infiltration in submaxillary gland and parotid gland, respectively, in TRAF3 mice. (H,I) H&E staining of parotid glands with pleomorphic adenoma and sarcoma, respectively. (J) Papillary bronchioloalveolar carcinoma of lung in a TRAF3 mouse. (K) H&E staining of hepatocellular carcinoma in TRAF3 mouse. Note the presence of subcapsular fat necrosis with chronic inflammatory cells. Bars (D-K), 100 μm.