Example of p73 real-time bisulfite PCR. Hypermethylated p73 gene promoter region DNA detected by use of the ABI Prism 7000 Sequence Detection System (TaqMan). (A) Example of a patient with hypermethylated p73. (B) Example of a case without p73 methylation. p73 methylation was estimated in relation to the detection of the interferon-γ gene (IFNG). ΔRn is defined as the cycle-to-cycle change in the reporter fluorescence signal normalized to a passive reference fluorescence signal (log scale). The horizontal line represents the threshold. CT indicates cycle threshold, the cycle number at which the Rn exceeds the baseline by the value of the threshold.