Dynamics of global DNA methylation pretreatment and at the time of remission documentation. Global DNA methylation was studied using the LINE bisulfite pyrosequencing assay as described. No differences in LINE methylation were observed pretreatment and at the time of remission (P = .1). Lines connect individual patient LINE methylation values pretreatment and at remission. The figures indicate mean methylation levels and ranges. This indicates that gene-specific methylation changes described herein are not the result of decreased leukemia burden.