Figure 5
![Figure 5. Growth factors and coculture with BMSCs do not protect against tunicamycin-induced BCWM.1 cell cytotoxicity. (A) BCWM.1 cells were cultured with control media, and with tunicamycin (0.5 μg/mL to 1 μg/mL) for 48 hours, in the presence or absence of BMSCs. Cell proliferation was assessed using the [3H]-thymidine uptake assay. (B) BCWM.1 cells were cultured with control media in the presence or absence of IL-6 (25 ng/mL), CD40L (3 ng/mL), or BLyS (100 ng/mL), and treated with tunicamycin (0.1 μg/mL and 1 μg/mL) for 48 hours. Cytotoxicity was assessed by the MTT assay. All data represent mean plus or minus SD of triplicate experiments (A,B).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/113/3/10.1182_blood-2007-10-116848/5/zh80040930010005.jpeg?Expires=1735565927&Signature=FCLP4ePlqXe7mGQat5vtxY3p0c1ptZ4iLukLxyDRHpjO5CsCTJc8og6ZUYmjD~oAFHkJEbj1agKZkhT9FUvuvzETSD6A5AA7nVeA0IPulZTYAEgPx1bIjCOxKMm1Jpu-NStYep1DzujXi-HihIU~HeHF~ae1e7F0jKoFKyv9sRvt8jijBSsFmFb8683nFSy69pxkZm0z1DO2fMW4pRtPxPc2gKfzaEZvCtu4Xz0gVAw4Cc1gyDs5731enrdqRtvn~t8A69hPHL9eW627zeMxMw~G~U2Q1obB8Gbmgdv9TlIe1Rei5P~BMAlZSrMHbYS0t9IdpT-DnsrNYyfoiZcWAQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Growth factors and coculture with BMSCs do not protect against tunicamycin-induced BCWM.1 cell cytotoxicity. (A) BCWM.1 cells were cultured with control media, and with tunicamycin (0.5 μg/mL to 1 μg/mL) for 48 hours, in the presence or absence of BMSCs. Cell proliferation was assessed using the [3H]-thymidine uptake assay. (B) BCWM.1 cells were cultured with control media in the presence or absence of IL-6 (25 ng/mL), CD40L (3 ng/mL), or BLyS (100 ng/mL), and treated with tunicamycin (0.1 μg/mL and 1 μg/mL) for 48 hours. Cytotoxicity was assessed by the MTT assay. All data represent mean plus or minus SD of triplicate experiments (A,B).
