Effect of 2-DG treatment on glucose consumption and prednisolone- induced cytotoxicity in human ALL cell lines. Graphic representation of relative glucose consumption (A) or in vitro prednisolone responsiveness (B) in 2 prednisolone-resistant and 2 prednisolone-sensitive ALL cell lines after 2-DG treatment. Glucose consumption was calculated by measuring the conversion of glucose to 6-phosphogluconate, and glucose consumption in Jurkat cells was set at 100%. Response to prednisolone was measured by the MTT assay; cell survival in nontreated cells was set at 100%. Used concentrations of prednisolone and 2-DG varied depending on cellular toxicity (550 μg/mL and 0.078 μg/mL prednisolone, and 2 mM and 0.5 mM for -resistant and -sensitive cell lines, respectively). Representative experiments are shown; data are presented as means plus or minus SD (n = 3).