Effect of glycolytic inhibitors on prednisolone-induced cytotoxicity in prednisolone–resistant and prednisolone-sensitive cell lines. Cell survival curves representing in vitro prednisolone responsiveness, as assessed by the MTT assay in 2 prednisolone-resistant (top panel) and 2 prednisolone-sensitive ALL cell lines (bottom panel) after treatment with glycolytic inhibitors. Cells were treated with 1 mM (Jurkat, Molt4) or 0.5 mM 2-DG (▲), 62.5 μM LND (), 30 μM 3-BrPA (●) in combination with prednisolone, as indicated in the figure. Cells treated only with prednisolone served as controls (■). To visualize synergy, the survival rate of prednisolone in combination with an inhibitor was corrected for the toxicity caused by the inhibitor itself (set to 100%). A representative experiment is shown; data are presented as means plus or minus SD (n = 3).