Phenotype analysis of splenic eGFP-specific CD8⁺ T cells. (A) 56 days after rAAVeGFP (top panel) or rAdeGFP (bottom panel) administration, splenocytes were costained with the eGFP_200-208 tetramer and antibodies to CD8, CD44, CD62L, CD127, and CD43 activation–associated glycoform (CD43 a.a.g.). Numbers represent percentage of tetramer⁺ CD8⁺ T cells that stain positive for each cell surface molecule. Gated as in Figure 1D. Data represent 2 independent experiments with 5 to 8 individual animals per group. (B) Scatter plot representing eGFP_200-208 tetramer and antibody staining to cell surface markers CD44, CD62L, CD127 and the CD43 activation–associated glycoform on splenocytes isolated from individual rAAVeGFP-treated and rAdeGFP-treated mice at day 56. Each dot represents the frequency of eGFP-tetramer⁺ cells that stain positive for each cell surface marker for an individual mouse. Statistical differences in surface marker expression between the rAdeGFP and rAAVeGFP-primed cells were analyzed using the Student t test.