Analysis of CD8⁺ T cells in quadriceps muscle positive for β-galactosidase. (A) Enzymatic detection of β-galactosidase in rAAVβgal-injected (left) and contralateral (right) quadriceps muscle 168 days after vector administration. Bar represents 100 μm. (B) β-Galactosidase was quantified in muscle by antigen-capture ELISA 56 days after rAAVβ-gal inoculation. (C) Numbers represent the percentage of CD8⁺ T cells that costain with the H-2K^b βgal_96-103 tetramer in spleen, liver and muscle of mice injected with rAAVβgal 56 days earlier. Plots gated as in Figure 1D. (D) Annexin V binding by H-2K^b βgal_96-103 tetramer⁺ CD8⁺ T lymphocytes from quadriceps muscle, liver, and spleen 56 days after transduction with rAAVβgal. Numbers represent the percentage of tetramer⁺ cells that bind annexin V. Dot plots gated as in Figure 4A (right panel). Data represent 2 independent experiments each with tissues pooled from 6 mice.