IL-17RB (IL-25 receptor) and cytokine expression by L-HES CD3⁻CD4⁺ T cells. (A) Four-color immunofluorescent labeling of control and P3's PBMCs. The lymphocyte populations were gated on CD4 and CD3 positivity/negativity. (B) Purified CD3⁻CD4⁺ T cells from P3 were cultured for 48 hours with phorbol ester and anti-CD28 in the absence or presence of increasing concentrations of rhIL-25, and cytokine concentrations were determined using BD Cytometric Bead Array Flex Sets. A representative experiment is shown.