Validation of changes in gene expression using quantitative RT-PCR and flow cytometry. (A-B) Fold change in the expression of selected genes measured by quantitative RT-PCR for (A) patients (P1-P3) relative to controls⁴  and (B) P1-yr +6 relative to P1-yr 0. P values were calculated based on 3 independent experiments using the Student t test and are indicated in the corresponding bar. (C) Histograms showing the surface expression of CD29 (ITGB1), CD49D (ITGA4), and CD62L (SELL) on control CD3⁺CD4⁺CD45RO⁺ T cells and P1-yr 0, P1-yr +4, and P1-yr +6 CD3⁻CD4⁺CD45RO⁺ T cells. Isotype controls (not shown) for each sample were set between 10⁰ and 10¹.