Figure 1
Figure 1. B-cell development in TAK1-deficient mice. (A) Western blot analysis of TAK1 deletion in mature B cells. Splenic mature B cells were isolated from 2 pairs of CD19CreTak1+/+ and CD19CreTak1fl/fl mice, and total cell lysates were subjected to Western blot analysis with anti-TAK1 antibody. Anti-actin immunoblotting was used as a protein loading control. (B) The numbers of total B cells and B-cell subpopulations in BM of TAK1-deficient mice. Histograms show the numbers of total BM cells, total B cells, pro/pre-, and immature and mature B cells in BM of CD19CreTak1+/+ and CD19CreTak1fl/fl mice. (C) B-cell subpopulations in BM of TAK1-deficient mice. BM cells from CD19CreTak1+/+ and CD19CreTak1fl/fl mice were stained with anti-B220 and anti-IgM antibodies. Percentages indicate cells in the gated lymphoid population. (D) Pro- and pre-B populations in BM of Tak1-deficient mice. BM cells from CD19CreTak1+/+ and CD19CreTak1fl/fl mice were stained with anti-B220, anti-CD43, and anti-IgM antibodies. Percentages indicate cells in the gated B220+IgM− population. (E) Reduction of total splenocytes and total B cells in the spleens of TAK1-deficient mice. Histograms show the numbers of total splenocytes, B220+ B cells and Thy1.2+ T cells in the spleens of CD19CreTak1+/+ and CD19CreTak1fl/fl mice. (F) Reduction of B-cell populations in the spleen of TAK1-deficient mice. Splenocytes from CD19CreTak1+/+ and CD19CreTak1fl/fl mice were stained with anti-B220 and anti-Thy1.2 antibodies. Percentages indicate cells in the gated lymphoid population. (G) Reduction of FO and MZ B cells in the spleens of TAK1-deficient mice. Splenocytes from CD19CreTak1+/+ and CD19CreTak1fl/fl mice were stained with antibodies to IgM, CD21, and CD23. In CD23+-gated cells, T2 B cells (CD21hiIgMhi) and FO B cells (CD21intIgMlo) are shown. In CD23−-gated cells, MZ B cells (CD21hiIgMhi) and T1 B cells (CD21loIgMhi) are shown. The percentages of cells in the gated lymphoid populations are indicated. (H) Reduction of T2, FO, and MZ B cell numbers in the spleens of TAK1-deficient mice. Histograms show the numbers of T1, T2, FO, and MZ B cells in the spleens of CD19CreTak1+/+ and CD19CreTak1fl/fl mice. (I) Reduction of FO B cells in the spleens of TAK1-deficient mice. Splenocytes from CD19CreTak1+/+ and CD19CreTak1fl/fl mice were stained with antibodies to B220, CD21, CD23, and CD24. In B220+CD23+-gated cells, FO (CD21intCD24int), T2FO (CD21intCD24hi), and T2MZP (CD21hiCD24hi) B cells are shown. Percentages indicate cells in the gated lymphoid population. Data shown are obtained from or representative of 7 (B-D), 8 (E-H), or 5 (I) 8- to 12-week-old mice of each genotype.

B-cell development in TAK1-deficient mice. (A) Western blot analysis of TAK1 deletion in mature B cells. Splenic mature B cells were isolated from 2 pairs of CD19CreTak1+/+ and CD19CreTak1fl/fl mice, and total cell lysates were subjected to Western blot analysis with anti-TAK1 antibody. Anti-actin immunoblotting was used as a protein loading control. (B) The numbers of total B cells and B-cell subpopulations in BM of TAK1-deficient mice. Histograms show the numbers of total BM cells, total B cells, pro/pre-, and immature and mature B cells in BM of CD19CreTak1+/+ and CD19CreTak1fl/fl mice. (C) B-cell subpopulations in BM of TAK1-deficient mice. BM cells from CD19CreTak1+/+ and CD19CreTak1fl/fl mice were stained with anti-B220 and anti-IgM antibodies. Percentages indicate cells in the gated lymphoid population. (D) Pro- and pre-B populations in BM of Tak1-deficient mice. BM cells from CD19CreTak1+/+ and CD19CreTak1fl/fl mice were stained with anti-B220, anti-CD43, and anti-IgM antibodies. Percentages indicate cells in the gated B220+IgM− population. (E) Reduction of total splenocytes and total B cells in the spleens of TAK1-deficient mice. Histograms show the numbers of total splenocytes, B220+ B cells and Thy1.2+ T cells in the spleens of CD19CreTak1+/+ and CD19CreTak1fl/fl mice. (F) Reduction of B-cell populations in the spleen of TAK1-deficient mice. Splenocytes from CD19CreTak1+/+ and CD19CreTak1fl/fl mice were stained with anti-B220 and anti-Thy1.2 antibodies. Percentages indicate cells in the gated lymphoid population. (G) Reduction of FO and MZ B cells in the spleens of TAK1-deficient mice. Splenocytes from CD19CreTak1+/+ and CD19CreTak1fl/fl mice were stained with antibodies to IgM, CD21, and CD23. In CD23+-gated cells, T2 B cells (CD21hiIgMhi) and FO B cells (CD21intIgMlo) are shown. In CD23−-gated cells, MZ B cells (CD21hiIgMhi) and T1 B cells (CD21loIgMhi) are shown. The percentages of cells in the gated lymphoid populations are indicated. (H) Reduction of T2, FO, and MZ B cell numbers in the spleens of TAK1-deficient mice. Histograms show the numbers of T1, T2, FO, and MZ B cells in the spleens of CD19CreTak1+/+ and CD19CreTak1fl/fl mice. (I) Reduction of FO B cells in the spleens of TAK1-deficient mice. Splenocytes from CD19CreTak1+/+ and CD19CreTak1fl/fl mice were stained with antibodies to B220, CD21, CD23, and CD24. In B220+CD23+-gated cells, FO (CD21intCD24int), T2FO (CD21intCD24hi), and T2MZP (CD21hiCD24hi) B cells are shown. Percentages indicate cells in the gated lymphoid population. Data shown are obtained from or representative of 7 (B-D), 8 (E-H), or 5 (I) 8- to 12-week-old mice of each genotype.

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