Thrombus formation in GPIIb and FVIII MO-injected fish in a laser-induced arterial injury model. (A) Representative phenotypes of 3 dpf control siblings (i) and fish injected with 2 ng GPIIb MO (itga2b atg1, ii), 4 ng FVIII MO (f8 sp2, iii), or coinjected with both MOs at 2 ng and 8 ng, respectively (iv). An intracranial hemorrhage, as observed in 5% of larvae, is indicated by arrowhead (iv). (B) Representative images of thrombus formation (arrows) 2 minutes after injury in 3-dpf wild-type larvae (i) or larvae injected with 2 ng GPIIb (ii) or 4 ng FVIII MOs (iii). CA indicates caudal artery; CV, caudal vein. White lines represent each thrombus as carried out in ImageJ for determining TSA. (C) Representative images of thrombus formation in 4-dpf CD41-GFP fish controls (i), and siblings injected with 2 ng GPIIb (ii) or 4 ng FVIII (iii) MOs showing thrombocyte-rich thrombi. (D) TTA and (E) TSA (2 minutes after injury) were measured and compared with uninjected sibling controls. Statistical analyses were performed using an unpaired Student t test (*P < .05; ***P < .001).