Effects of antisense knockdown of ANTXR2, BAMBI, DCBLD2, ESAM, and LRRC32 zebrafish orthologs on thrombus formation. TTA (A) and TSA (B) in the MO-injected fish were compared with that of their matched sibling controls. Each experiment is representative of more than 4 experiments, including similar numbers for both groups, using 2 nonoverlapping MOs. Zebrafish larvae were injected with the following MOs: 2 ng ANTXR2 atg1, 2 ng ANTXR2 sp1, 2 ng BAMBI atg1, 1 ng BAMBI sp1, 4 ng DCBLD2 atg1, 0.5 ng DCBLD2 sp1, 1 ng ESAM sp1, 12 ng ESAM sp2, 4 ng LRRC32 atg1, or 2 ng LRRC32 sp1. Statistical analyses were performed using an unpaired Student t test (*P < .05; **P < .005). (C) Results from all experiments and for each of the 2 MOs tested per gene were combined for TTA (□) and TSA () using Cox regression and linear mixed modeling, respectively, as described in “Statistical analysis of the thrombosis model” (*P < .05; **P < .005; ***P < .001). †TTA data for each DCBLD2 MO were significantly different and therefore were not combined. ††Results for TSA are applicable for esam sp1 only. Detailed P values for the combined and individual MO analysis are presented in Table 3.