Antagonistic effects of sJAM-A on endothelial cells. (A,B) A confluent HUVEC layer was wounded by a defined scratch; and after of 24 hours of incubation in the presence or absence of dimeric sJAM-A.Fc or human IgG (both 5 and 10 μg/mL), endothelial cells that had migrated into the scratch area were counted. (A) A representative experiment. (B) Mean plus or minus SD values were calculated for 3 independent experiments. *Statistically significant changes in endothelial migration compared with the control receiving hIgG (P < .05).