Reconstitution potential of JAM-C+ BM cells. (A) A total of 500 JAM-C−, JAM-C+, and LSK cells were sorted from the BM of C57BL6 donor mice and injected intravenously into lethally irradiated BL6/SJL recipients along with 2 × 105 host-type BM cells for rescue. Peripheral blood was stained with anti-CD45.2 for identification of donor progeny and shown as the frequency of donor-derived cells after transfer. Each line represents the frequency of donor-derived cells in a single mouse. The experiment was repeated 3 times with an average of 5 animals per group. (B) The presence of donor-derived cells within the different blood cell lineages was determined by gating on B220+ (B cells), CD3ϵ+ (T cells), or CD11b+/Gr-1+ (granulocytes) prior to the assessment of the frequency of CD45.2+ cells, LSK cells (▴), JAM-C+ (—●—), and JAM-C− (- - -●- - -). Shown is the average of all mice of 3 independent experiments with an average of 5 animals per group that showed reconstitution above background level as determined by analysis of B6/SJL mice that did not receive transplants (≥ 0.4% B220+, and > 0% CD3+ and CD11b/Gr-1+). Significances are shown on the graph. **P ≤ .01.
