Effects of Jam-C deletion on hematopoietic progenitor numbers and activities. Assessment of the frequency (A,B) and total number (B) of lineage-negative cells in the BM of wild-type and homozygous (n = 7) mice. (C) Flow cytometric analysis of LT- and ST-HSCs (Lin⁻, Sca-1⁺, c-Kit⁺Thy1.2⁺) and progenitors (MPPs: Lin⁻, Sca-1⁺, c-Kit⁺Thy1.2⁻; CMPs: Lin⁻, Sca-1⁻, c-Kit⁺; CLPs: Lin⁻, Sca-1^int, c-Kit^int) contained within the Lin⁻ population. Frequency (D) and total number (E) of stem cells and progenitors in the BM of wild-type and homozygous mice. Shown are representative FACS profiles; the numbers on the plots indicate the frequency of cells in the indicated regions. Bar graphs indicate mean plus or minus SE. (F) Colony-forming ability of BM cells derived from wild-type (n = 5) and homozygous (n = 6) mice cultured in methylcellulose containing mSCF, mIL-3, mIL-6, and hEPO. After 10 days in culture, colonies were assigned scores for the presence of CFU-GEMM, BFU-E, and CFU-GM. Data represent mean plus or minus SE of duplicate plates. Significances are shown on the graph. *P ≤ .05.