Human apo A-I gene transfer and EPC number in chimeric C57BL/6 SR-BI^+/+ mice and chimeric C57BL/6 SR-BI^−/− mice. (A) Human apo A-I expression levels after adenoviral gene transfer with 5 × 10¹⁰ particles of AdA-I in male C57BL/6 mice transplanted with SR-BI^+/+ (●; n = 10) or SR-BI^−/− (▲; n = 10) bone marrow 4 weeks before gene transfer. (B) Time course of the number of Flk-1 Sca-1 double-positive cells in AdA-I (●, ▲)– or Adnull (○, △)–treated C57BL/6 mice with SR-BI^+/+ (●, ○) or SR-BI^−/− (▲, △) bone marrow (n = 10 for each group). (C) Bar graph showing the number of DiI-acLDL FITC-isolectin double-positive cells after 7 days of ex vivo culture of bone marrow mononuclear cells isolated at day 35 after Adnull transfer or AdA-I transfer in C57BL/6 mice with SR-BI^+/+ or SR-BI^−/− bone marrow (n = 10 for each group). (D) Bar graph showing the number of Flk-1 Sca-1 double-positive cells in the bone marrow of C57BL/6 mice with SR-BI^+/+ or SR-BI^−/− bone marrow at day 35 after transfer with Adnull or AdA-I. Data are expressed as number/10 000 mononuclear cells (MNC) (n = 10 for each group). Data are mean plus or minus SEM.