Identification of macrophages as main target for VWF in spleen and liver. (A,B) Spleen sections of VWF-deficient mice treated with recombinant VWF (10 μg/mouse). Spleen was collected 30 minutes after injection. Sections are stained using polyclonal anti-VWF antibodies (A) or the anti–mouse macrophage marker F4/80 (B), and counterstained using hematoxylin (magnification 200×). RP indicates red pulp; WP, white pulp. (C) Merged image of a spleen tissue section stained with anti–mouse F4/80 antibody (red) and polyclonal antihuman antibodies VWF (green). Original magnification 400×. (D,E) Liver sections of VWF-deficient mice treated with recombinant VWF (10 μg/mouse). Liver was collected 30 minutes after injection. Sections are stained using polyclonal anti-VWF antibodies (D) or a monoclonal anti–mouse CD68 antibody (E), and counterstained using hematoxylin (magnification 200×). (F) Merged image of liver tissue section stained with anti–mouse CD68 antibody (red) and polyclonal anti–human VWF antibodies (green). Original magnification 400×. Red arrowheads in panels D and E point to examples of positively stained cells (brown color).