Increased VWF and FVIII levels upon gadolinium chloride treatment. (A) Liver sections of wild-type mice treated with saline or GdCl₃ were stained with antimouse CD68. Kupffer cells were identified as CD68⁺ cells with an appropriate nuclear morphology and sinusoidal location. Cells were counted from at least 10 randomly selected fields per section. One field corresponds to a surface of 0.1 mm². (B,C) VWF-deficient mice (B) or wild-type mice (C) were treated with saline or GdCl₃, and 24 to 48 hours after treatment plasma samples were taken. Samples were analyzed for FVIII activity (B) or VWF antigen (C). (D) VWF-deficient mice were treated with saline (•) or GdCl₃ (○) 24 hours prior to intravenous injection with VWF (5 μg/mouse). At indicated time points, samples were drawn and analyzed for residual VWF antigen. For clarity, data for the first 8 hours are shown. Calculation of MRT values was based on data obtained over a 24-hour period. (A-D) Data represent mean plus or minus SD of 3 to 9 experiments. *P < .05; **P < .001.