Effect(s) of CD40/IL-4 and HCV on B-cell adhesion to Huh-7.5 hepatoma cells. Untreated and CD40L/IL-4–stimulated L3055-Bcl-2 (□) and PBMC-derived () B cells were labeled with CMFDA and added to a confluent monolayer of Huh-7.5 cells for 15 minutes at 37°C. Nonbound cells were removed by gentle washing, and the adherent cells were enumerated (A). Alternatively, PBMC-derived B cells from 7 healthy donors were incubated with mock media or JFH-1 overnight, labeled with CMFDA, and quantified for their adhesion to Huh-7.5 cells (B). The data are presented as adhered lymphocytes per 10 000 Huh-7.5 cells. The first panel depicts the median values for mock (○) and HCV-exposed B cells (●), and the second panel indicates the response per donor and shows a significant increase in binding after HCV exposure (P = .0156, nonparametric paired t test [Wilcoxon]).