Increased HNE and TSP2 staining in CYP1B1^−/− mice retina. Frozen eye sections prepared from P17 CYP1B1^+/+ (A,C,E,G) and CYP1B1^−/− mice (B,D,F,H) exposed to OIR receiving solvent control (A,B,E,F) or NAC (C,D,G,H; 10 mg/kg in 0.1 mL IP) from P12 to P17, were stained with specific antibodies to HNE (A-D) and TSP2 (E-H) (×200). Please note the marked fluorescence staining for TSP2 and HNE (arrowheads) in CYP1B1^−/− retina which are diminished upon administration of NAC compared with CYP1B1^+/+ retinas. Sections were treated identically and images were obtained under identical conditions. These experiments were repeated twice with eyes from 4 different mice with similar results.