ALMC-1 and ALMC-2 cells exhibit c-myc amplification and a 14;20 translocation. (A) Representative cIg-FISH image of ALMC-1 cells showing c-MYC amplification. (B) Representative interphase FISH image of ALMC-2 cells demonstrating a 14;20 translocation. The fusion signal (arrow) resulting from comigration of the IGH and MAFB probes is consistent with a t(14;20). Original magnification ×100 (Leica DMRXA microscope, Wetzlar, Germany) for panels A and B. (C) MAFB expression levels in ALMC-1 and ALMC-2 cell lines relative to other MM cell lines that do or do not express a t(14;20). (D) MAFB expression levels in primary patient tumor cells relative to a number of other primary patient MM cells.