Swapping HVR5 and 7 of Ad5 to Ad26 and its effect on FX binding by SPR. (A) Viewlite images of the Ad5 hexon showing HVR5 (blue), HVR7 (green), as well as the remaining HVR amino acids (gray). (B) Virus particle titers and VP/pfu ratios for each virus produced. (C) SPR analysis of virus interaction with immobilized FX (500 RU). Sensorgrams of 10¹¹ VP/mL injected at a flow rate of 30 μL/minute.