Central and peripheral hematopoietic reconstitution by AKT1^−/−AKT2^−/− FL-derived HSCs. The frequency of (A) LSK cells in the lineage-negative gate or (B) LT-HSCs (CD150⁺CD48⁻LSKs) in the total LSK gate of E14.5 FLs. Graphs represent the mean ± SEM (n = 5). (C) The total number of colonies generated 10 days after equal numbers of FL cells were plated in methylcellulose media. The diversity of colonies was not different between the 2 genotypes. Data are represented as the percentage of wild-type colonies generated within each experiment and represent the mean ± SEM (n = 4). The frequency of (D) LSK cells in the lineage-negative gate or (E) LT-HSCs (CD150⁺CD48⁻LSKs) in the total LSK gate of bone marrow from irradiated mice reconstituted 12 weeks previously with FL cells of the indicated genotype. Graphs represent the mean ± SEM (n = 3). (F) The total number of cells in the splenic myeloid (Mac-1⁺Gr-1⁺), B (B220⁺), and T (TCRβ⁺) lineages in mice from panels D and E. Graphs represent the mean ± SEM (n = 3).