Apoptosis and proliferation analysis after pre-miR-135 transfection in the L-428 cell line. (A) Analysis at 48 hours after transfection showed that caspase 3/caspase 7 activity was 36% higher than in control cells, transfected with pre–miR-negative control (**P < .01). (B) The proliferation assay at 72 hours showed that pre–miR-135–transfected cells grew less than control cells transfected with pre-miRNA precursor-negative control (*P < .05).