Bcl-xL expression analysis. (A) Reverse-transcribed polymerase chain reaction of Bcl-xL at 72 hours after transfection with 100 nM of pre-miR-135a. A reduction in Bcl-xL mRNA levels can be observed in the pre–miR-135–transfected cells versus the control cells. No change was observed in the β-glucuronidase (GUSB) mRNA levels, used as control. (B) Western blot analysis of Bcl-xL at 72 hours after transfection showed a correlation between BcL-xL mRNA and protein levels.