Intracellular distribution of wild-type and mutants of HTLV-1–Tax protein in transiently transfected Jurkat cells. Jurkat cells were transfected with GFP-Tax or GFP-Tax mutants M7, M22, or M47. Jurkat cells were fixed with cold methanol (6 hours after transfection) and permeabilized. The MTOC was stained with polyclonal anti–γ-tubulin antibody (red). Each panel shows projected images (x, z) corresponding to GFP-Tax or GFP-Tax mutant (green), MTOC (red), and the superposition of the 2 colors (merge). Scale bar represents 10 μm.